VIABILITY OF VERO E6 CELLS EXPOSED TO THE Bothrops leucurus SNAKE CRUDE VENOM

  • Jacqueline Ramos Machado Braga Universidade Federal do Recôncavo da Bahia
  • Bruna dos Santos Bispo Universidade Federal do Recôncavo da Bahia
  • Victor Lucas Dias Cavalcante Universidade Federal do Recôncavo da Bahia
  • Silvia Inês Sardi Universidade Federal da Bahia
  • Gúbio Soares Campos Universidade Federal da Bahia
  • Ilka Biondi Universidade Estadual de Feira de Santana
  • Luis Eduardo Meira Faria Universidade Federal do Recôncavo da Bahia
DOI: https://doi.org/10.15210/sah.v6i3.14231

Resumo

Primary and continuous cell culture systems are a good in vitro alternative for the quantitative assessment of snake venom toxicity. There are still few studies evaluating the cellular response against Bothrops leucurus venom (BlV). This study aimed to evaluate the cytotoxic effect of crude BlV on culture of VERO E6 cells. Neutral Red incorporation (NR) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) tests were performed for viable cells, and Trypan Blue (TB) dye exclusion test was performed for non-viable cells. The differences between the groups were evaluated by ANOVA-One-Way and Dunnet through the program Graph Pad 5.0 with significance of 5%. The 50% cytotoxic concentration (CC50) of BlV was 7.86 μg/mL. There was a trend of dose-dependent reduction on cell viability in all assays evaluated. However, MTT and NR tests were more sensitive for the evaluation of BlV cytotoxicity on the VERO E6 cell line.

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Publicado
2019-07-23
Como Citar
Ramos Machado Braga, J., dos Santos Bispo, B., Dias Cavalcante, V. L., Sardi, S. I., Soares Campos, G., Biondi, I., & Meira Faria, L. E. (2019). VIABILITY OF VERO E6 CELLS EXPOSED TO THE Bothrops leucurus SNAKE CRUDE VENOM. Science and Animal Health, 6(3), 190-206. https://doi.org/10.15210/sah.v6i3.14231
Edição
v. 6 n. 3 (2018)
Seção
Ciência Veterinária Básica